| 1 | 1 | Hannaford Bros | 7,501–8,000 | Supermarkets |
| 2 | 2 | Walmart/Sam’s Club | 7,001–7,500 | Discount department stores |
| 2 | 3 | Maine Medical Center | 6,501–7,000 | General medical/surgical hospitals |
| 4 | 4 | Bath Iron Works | 5,001–5,500 | Ship building and repairing |
| 3 | 5 | L.L.Bean, Inc. | 4,001–4,500 | Mail order houses |
| 6 | 6 | Eastern Maine Medical Center | 3,501–4,000 | General medical/surgical hospitals |
| 10 | 7 | MaineGeneral Medical Center | 3,001–3,500 | General medical/surgical hospitals |
| 9 | 8 | Central Maine Healthcare | 3,001–3,500 | General medical/surgical hospitals |
| 7 | 9 | TD Bank | 2,501–3,000 | Commercial banking |
| 8 | 10 | Unum Provident | 2,501–3,000 | Direct life insurance carriers |
| 11 | 11 | Shaw’s Supermarkets | 2,001–2,500 | Supermarkets |
| 18 | 12 | Webber Hospital Association | 1,501–2,000 | General medical/surgical hospitals |
| 12 | 13 | Mercy Hospital | 1,501–2,000 | General medical/surgical hospitals |
| 15 | 14 | Home Depot | 1,501–2,000 | Home centers |
| 20 | 15 | Lowe’s Home Centers | 1,001–1,500 | Home centers |
| 13 | 16 | Verso Paper | 1,001–1,500 | Paper, except newsprint, mills |
| 22 | 17 | Goodwill Industries | 1,001–1,500 | Vocational rehabilitation services |
| 17 | 18 | S. D. Warren | 1,001–1,500 | Paper, except newsprint, mills |
| 32 | 19 | Circle K | 1,001–1,500 | Gasoline stations and convenience stores |
| 14 | 20 | Pratt & Whitney Aircraft | 1,001–1,500 | Aircraft engine and engine parts manufacturing |
| 16 | 21 | St. Mary’s Regional Medical Center | 1,001–1,500 | General medical and surgical hospitals |
| 19 | 22 | Rite Aid of Maine | 1,001–1,500 | Pharmacies and drug stores |
| 21 | 23 | Jackson Laboratory | 1,001–1,500 | Research and development, biotechnology |
| 23 | 24 | Bowdoin College | 1,001–1,500 | Colleges and universities |
| 25 | 25 | University of New England | 1,001–1,500 | Colleges and universities |
| 27 | 26 | Mid Coast Hospital | 1,001–1,500 | General medical/surgical hospitals |
| NL | 27 | Maine Behavioral Health Center | 1,001–1,500 | Outpatient mental health centers |
| 26 | 28 | York Hospital | 1,001–1,500 | General medical and surgical hospitals |
| 35 | 29 | IDEXX Laboratories | 1,001–1,500 | Pharmaceutical preparation manufacturing |
| NL | 30 | Aroostook Medical Center | 1,001–1,500 | General medical/surgical hospitals |
| NL | 31 | Penobscot Bay Medical Center | 501–1,000 | General medical/surgical hospitals |
| 34 | 32 | Central Maine Power Company | 501–1,000 | Electric power distribution |
| NL | 33 | New Balance Shoe | 501–1,000 | Footwear manufacturing |
| 40 | 34 | United Parcel Service | 501–1,000 | Couriers and express delivery services |
| 33 | 35 | Spurwink Services | 501–1,000 | Individual and family services |
| 36 | 36 | Bates College | 501–1,000 | Colleges and universities |
| 39 | 37 | Attendant Services | 501–1,000 | Services for elderly and disabled people |
| 43 | 38 | Marden’s | 501–1,000 | Discount department stores |
| 47 | 39 | C. N. Brown | 501–1,000 | Fuel dealers |
| 50 | 40 | St. Joseph Hospital | 501–1,000 | General medical/surgical hospitals |
| 42 | 41 | North Country Associates | 501–1,000 | Nursing care and skilled nursing |
| 46 | 42 | Cianbro Corporation | 501–1,000 | Highway, road, and bridge construction |
| 48 | 43 | Colby College | 501–1,000 | Colleges and universities |
| NL | 44 | FIA Card Services | 501–1,000 | Credit card issuing |
| 29 | 45 | Dead River Company | 501–1,000 | Fuel dealers |
| NL | 46 | Time Warner | 501–1,000 | Wired telecommunications carriers |
| NL | 47 | Pine State Trading Company | 501–1,000 | Wine and spirit wholesalers |
| 41 | 48 | Anthem Health Systems | 501–1,000 | Direct health and medical insurance |
| NL | 49 | Bonney Staffing Center | 501–1,000 | Temporary help services |
| NL | 50 | Manpower | 501–1,000 | Temporary help services |